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202019 addgene 140989 software  (Addgene inc)


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    Structured Review

    Addgene inc 202019 addgene 140989 software
    202019 Addgene 140989 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/202019+addgene+140989+software/pcDNA3%2E1-GGamma1-GFP2+(Plasmid+%23140989)/pm37148874-128-57-58
    Average 91 stars, based on 5 article reviews
    202019 addgene 140989 software - by Bioz Stars, 2026-11
    91/100 stars

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    Recombinant:

    Article Title: Molecular insights into peptide agonist engagement with the PTH receptor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Competent E. coli New England Biolabs C2527H Chemicals, peptides, and recombinant proteins PTH GL Biochem N/A PTHrP GL Biochem N/A ABL GL Biochem N/A M-PTH GL Biochem N/A LA-PTH Genscript N/A ESF 921 medium Expression Systems cat#96-001-01 cOmplete Protease Inhibitor Cocktail tablets Merck cat#11697498001 Apyrase New England Biolabs cat# M0398L Lauryl maltose neopentyl glycol Anatrace cat# NG310 Cholesteryl hemisuccinate Anatrace cat#CH210 Benzonase Millipore Sigma cat#71205 Flag peptide Merck Millipore cat#F3290 ANTI-FLAG M1 Agarose Affinity Gel Merck Millipore cat#A4596 Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) Millipore Sigma cat#UFC910024 Coelentazine-h Nanolight Tech cat#3031 Prolume purple Nanolight Tech cat#369 Forskolin Millipore Sigma cat#F6886 DMEM Thermo Fisher cat#11995065 HBSS Thermo Fisher cat#14065056 Critical commercial assays LANCE TR-FRET cAMP Perkin Elmer cat#TRF0262 Bac-to-Bac system Thermo Fisher cat#10359016 Deposited data PTH:PTH1R:Gs coordinates This paper PDB: 8FLQ PTH:PTH1R:Gs: EM maps This paper EMD: 29283 PTHrP:PTH1R:Gs coordinates This paper PDB: 8FLR PTHrP:PTH1R:Gs EM maps This paper EMD: 29284 ABL:PTH1R:Gs coordinates This paper PDB: 8FLS ABL:PTH1R:Gs EM maps This paper EMD: 29285 M-PTH:PTH1R:Gs coordinates This paper PDB: 8FLT M-PTH:PTH1R:Gs EM maps This paper EMD: 29286 LA-PTH:PTH1R:Gs coordinates This paper PDB: 8FLU LA-PTH:PTH1R:Gs EM maps This paper EMD: 29287 GLP-1:GLP-1R:Gs coordinates Yang et al., 201533 PDB: 6X18 Experimental models: Cell lines High Five Cells Thermo Fisher Scientific Cat#B85502 Cos-7 cells ATCC CRL-1651 HEK293A cells Thermo Fisher Scientific R70507 (Continued on next page) Structure 31, 668–676.e1–e5, June 1, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA HA-FLAG-3C-PTH1R-3C-His (pFastbac dual) This paper N/A HA-FLAG-3C-PTH1R-3C-His (pcDNA3.1) This paper N/A Gb1/Gg2 (pFastbac dual) This paper N/A Dominant Negative Gas (pFastbac) Liang et al., 201817 N/A Nb35 Rasmussen et al., 201118 N/A G⍺S-L-Rluc8 Olsen et al., 202019 Addgene 140981 Gb1 Olsen et al., 202019 Addgene 140987 g1-GFP Olsen et al., 202019 Addgene 140989 Software and algorithms EPU 2 Thermo Fisher Scientific https://www.thermofisher.com/au/en/ home/electron-microscopy/products/ software-em-3d-vis/epu-software.html EPU_Group_AFIS.py script GitHub https://github.com/DustinMorado/ EPU_group_AFIS RELION 3.1.2 Zivanov et al., 201847 https://github.com/3dem/relion crYOLO Wagner et al., 201948 cryolo.readthedocs.io/en/stable/ CryoSPARC Punjani et al., 201749 https://cryosparc.com/ UCSF ChimeraX Goddard et al., 201850 https://www.cgl.ucsf.edu/chimerax/ PyMol Schrödinger https://pymol.org/ Isolde Croll, 201851 https://isolde.cimr.cam.ac.uk/ Coot Emsley et al., 201052 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ PHENIX Liebschner et al., 201953 https://phenix-online.org/download/ LigPlot+ Laskowski and Swindells54 https://www.ebi.ac.uk/thornton-srv/ software/LigPlus/ Prism Graphpad https://www.graphpad.com/scientific- software/prism/ ..

    Dominant Negative Mutation:

    Article Title: Molecular insights into peptide agonist engagement with the PTH receptor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Competent E. coli New England Biolabs C2527H Chemicals, peptides, and recombinant proteins PTH GL Biochem N/A PTHrP GL Biochem N/A ABL GL Biochem N/A M-PTH GL Biochem N/A LA-PTH Genscript N/A ESF 921 medium Expression Systems cat#96-001-01 cOmplete Protease Inhibitor Cocktail tablets Merck cat#11697498001 Apyrase New England Biolabs cat# M0398L Lauryl maltose neopentyl glycol Anatrace cat# NG310 Cholesteryl hemisuccinate Anatrace cat#CH210 Benzonase Millipore Sigma cat#71205 Flag peptide Merck Millipore cat#F3290 ANTI-FLAG M1 Agarose Affinity Gel Merck Millipore cat#A4596 Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) Millipore Sigma cat#UFC910024 Coelentazine-h Nanolight Tech cat#3031 Prolume purple Nanolight Tech cat#369 Forskolin Millipore Sigma cat#F6886 DMEM Thermo Fisher cat#11995065 HBSS Thermo Fisher cat#14065056 Critical commercial assays LANCE TR-FRET cAMP Perkin Elmer cat#TRF0262 Bac-to-Bac system Thermo Fisher cat#10359016 Deposited data PTH:PTH1R:Gs coordinates This paper PDB: 8FLQ PTH:PTH1R:Gs: EM maps This paper EMD: 29283 PTHrP:PTH1R:Gs coordinates This paper PDB: 8FLR PTHrP:PTH1R:Gs EM maps This paper EMD: 29284 ABL:PTH1R:Gs coordinates This paper PDB: 8FLS ABL:PTH1R:Gs EM maps This paper EMD: 29285 M-PTH:PTH1R:Gs coordinates This paper PDB: 8FLT M-PTH:PTH1R:Gs EM maps This paper EMD: 29286 LA-PTH:PTH1R:Gs coordinates This paper PDB: 8FLU LA-PTH:PTH1R:Gs EM maps This paper EMD: 29287 GLP-1:GLP-1R:Gs coordinates Yang et al., 201533 PDB: 6X18 Experimental models: Cell lines High Five Cells Thermo Fisher Scientific Cat#B85502 Cos-7 cells ATCC CRL-1651 HEK293A cells Thermo Fisher Scientific R70507 (Continued on next page) Structure 31, 668–676.e1–e5, June 1, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA HA-FLAG-3C-PTH1R-3C-His (pFastbac dual) This paper N/A HA-FLAG-3C-PTH1R-3C-His (pcDNA3.1) This paper N/A Gb1/Gg2 (pFastbac dual) This paper N/A Dominant Negative Gas (pFastbac) Liang et al., 201817 N/A Nb35 Rasmussen et al., 201118 N/A G⍺S-L-Rluc8 Olsen et al., 202019 Addgene 140981 Gb1 Olsen et al., 202019 Addgene 140987 g1-GFP Olsen et al., 202019 Addgene 140989 Software and algorithms EPU 2 Thermo Fisher Scientific https://www.thermofisher.com/au/en/ home/electron-microscopy/products/ software-em-3d-vis/epu-software.html EPU_Group_AFIS.py script GitHub https://github.com/DustinMorado/ EPU_group_AFIS RELION 3.1.2 Zivanov et al., 201847 https://github.com/3dem/relion crYOLO Wagner et al., 201948 cryolo.readthedocs.io/en/stable/ CryoSPARC Punjani et al., 201749 https://cryosparc.com/ UCSF ChimeraX Goddard et al., 201850 https://www.cgl.ucsf.edu/chimerax/ PyMol Schrödinger https://pymol.org/ Isolde Croll, 201851 https://isolde.cimr.cam.ac.uk/ Coot Emsley et al., 201052 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ PHENIX Liebschner et al., 201953 https://phenix-online.org/download/ LigPlot+ Laskowski and Swindells54 https://www.ebi.ac.uk/thornton-srv/ software/LigPlus/ Prism Graphpad https://www.graphpad.com/scientific- software/prism/ ..

    Software:

    Article Title: Molecular insights into peptide agonist engagement with the PTH receptor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Competent E. coli New England Biolabs C2527H Chemicals, peptides, and recombinant proteins PTH GL Biochem N/A PTHrP GL Biochem N/A ABL GL Biochem N/A M-PTH GL Biochem N/A LA-PTH Genscript N/A ESF 921 medium Expression Systems cat#96-001-01 cOmplete Protease Inhibitor Cocktail tablets Merck cat#11697498001 Apyrase New England Biolabs cat# M0398L Lauryl maltose neopentyl glycol Anatrace cat# NG310 Cholesteryl hemisuccinate Anatrace cat#CH210 Benzonase Millipore Sigma cat#71205 Flag peptide Merck Millipore cat#F3290 ANTI-FLAG M1 Agarose Affinity Gel Merck Millipore cat#A4596 Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) Millipore Sigma cat#UFC910024 Coelentazine-h Nanolight Tech cat#3031 Prolume purple Nanolight Tech cat#369 Forskolin Millipore Sigma cat#F6886 DMEM Thermo Fisher cat#11995065 HBSS Thermo Fisher cat#14065056 Critical commercial assays LANCE TR-FRET cAMP Perkin Elmer cat#TRF0262 Bac-to-Bac system Thermo Fisher cat#10359016 Deposited data PTH:PTH1R:Gs coordinates This paper PDB: 8FLQ PTH:PTH1R:Gs: EM maps This paper EMD: 29283 PTHrP:PTH1R:Gs coordinates This paper PDB: 8FLR PTHrP:PTH1R:Gs EM maps This paper EMD: 29284 ABL:PTH1R:Gs coordinates This paper PDB: 8FLS ABL:PTH1R:Gs EM maps This paper EMD: 29285 M-PTH:PTH1R:Gs coordinates This paper PDB: 8FLT M-PTH:PTH1R:Gs EM maps This paper EMD: 29286 LA-PTH:PTH1R:Gs coordinates This paper PDB: 8FLU LA-PTH:PTH1R:Gs EM maps This paper EMD: 29287 GLP-1:GLP-1R:Gs coordinates Yang et al., 201533 PDB: 6X18 Experimental models: Cell lines High Five Cells Thermo Fisher Scientific Cat#B85502 Cos-7 cells ATCC CRL-1651 HEK293A cells Thermo Fisher Scientific R70507 (Continued on next page) Structure 31, 668–676.e1–e5, June 1, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA HA-FLAG-3C-PTH1R-3C-His (pFastbac dual) This paper N/A HA-FLAG-3C-PTH1R-3C-His (pcDNA3.1) This paper N/A Gb1/Gg2 (pFastbac dual) This paper N/A Dominant Negative Gas (pFastbac) Liang et al., 201817 N/A Nb35 Rasmussen et al., 201118 N/A G⍺S-L-Rluc8 Olsen et al., 202019 Addgene 140981 Gb1 Olsen et al., 202019 Addgene 140987 g1-GFP Olsen et al., 202019 Addgene 140989 Software and algorithms EPU 2 Thermo Fisher Scientific https://www.thermofisher.com/au/en/ home/electron-microscopy/products/ software-em-3d-vis/epu-software.html EPU_Group_AFIS.py script GitHub https://github.com/DustinMorado/ EPU_group_AFIS RELION 3.1.2 Zivanov et al., 201847 https://github.com/3dem/relion crYOLO Wagner et al., 201948 cryolo.readthedocs.io/en/stable/ CryoSPARC Punjani et al., 201749 https://cryosparc.com/ UCSF ChimeraX Goddard et al., 201850 https://www.cgl.ucsf.edu/chimerax/ PyMol Schrödinger https://pymol.org/ Isolde Croll, 201851 https://isolde.cimr.cam.ac.uk/ Coot Emsley et al., 201052 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ PHENIX Liebschner et al., 201953 https://phenix-online.org/download/ LigPlot+ Laskowski and Swindells54 https://www.ebi.ac.uk/thornton-srv/ software/LigPlus/ Prism Graphpad https://www.graphpad.com/scientific- software/prism/ ..



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    202019 addgene 140989 software  (Addgene inc)
    91
    Addgene inc 202019 addgene 140989 software
    202019 Addgene 140989 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/202019+addgene+140989+software/pcDNA3%2E1-GGamma1-GFP2+(Plasmid+%23140989)/pm37148874-128-57-58
    Average 91 stars, based on 1 article reviews
    202019 addgene 140989 software - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier

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